ZeTox – Toxicology Services

BY
OUR
PLATFORMS

icon-adme-bioavaility Bioavailability Assay

Absorption, distribution, metabolism, and excretion are the four main elements of ADME studies. Devoted to better understand the disposition of a pharmaceutical compound within an organism, ADME studies are influenced by pharmacokinetics and pharmacodynamics compounds properties and could be easily tested in zebrafish.

Advantages

Total control of the exposure medium (water).

Distribution and metabolism can be easily assessed in adult zebrafish.

Method description

We set up a methodology to analyze the amount of drug absorbed and distributed in the embryos. This can be applied also to specific tissues, and extended to production of metabolites and excretion. After incubation, whole embryos, or part of them, will be lyophilized for Mass Spec/NMR analysis. Drug content in bodies is calculated taking into account the initial amount of drug and the remaining amount in the exposure medium (water).

Readouts

Drug content concentrations in different tissues or organs  compared with concentrations shown in exposure medium.

References

  1. Chuenlei Parng. In vivo zebrafish assays for toxicity testing. Curr Opin Drug Discov Devel. 2005 Jan;8(1):100-6.
  2. Yoganantharjah and Gibert. The Use of the Zebrafish Model to Aid in Drug Discovery and Target Validation. Curr Top Med Chem. 2017;17(18):2041-2055.
  3. Zon et al. Use of Zebrafish in Drug Discovery Toxicology. Chem Res Toxicol. 2020 Jan 21; 33(1): 95–118.