CNS – Central Nervous System Models

An array of neurologic phenotypes comprising automated locomotion measurements, studies on neuronal morphology, and complex cognitive functions can be evaluated in a cost-effective and validated manner. Among vertebrates, the CNS is highly preserved, and zebrafish allows in vivo and non-invasive characterization thanks to its transparency.  Moreover, due to the large progenies, we are able to provide simultaneous assays/day on CNS-related studies.

Some examples of our capabilities are the study of behavioural alterations associated with Parkinson's or Epilepsy, or the study of thigmotaxis as a side effect.  Besides standardised disease modelling, we specialise in planning and validating customised solutions. Please contact us if you are in need of other readouts not present below.

Validation figure and phenotypes

Same as ZeEfficacy – Neurophenotyping models

  • Locomotion activity and visual response assay: Total distance moved, symptomatic movement patterns, light/dark or tapping stimuli responses.
  • Thigmotaxis: Evaluation of the anxiety state of the larvae after anxiogenic or anxiolytic treatment. The evaluation is based on both the total distance moved and the time spent in either centre or periphery of the well plate.
  • Habituation assay: Evaluation of innate learning through short memory. The parameter is represented by the locomotion pattern as a response to external stimuli also known as habituation phenotype.
  • Convulsive assay: Seizures can be evaluated as a response to a flashlight. These are evaluated through velocity and angle turn parameters.
  • Motoneuron cluster: Neuronal mortality and phenotyping via confocal GFP imaging of fixed larvae.

View our CNS – Central Nervous System Models services