CRISPR/Cas9 Knock-out

At ZeClinics we work with the original CRISPR/Cas9 license from the Broad Institute, MIT and Harvard University to provide the following solutions:

  • Single Knock-Out lines (mutation of one gene)
  • Double Knock-Out lines (simultaneous mutation of two genes, e.g. two paralogues)
  • CRISPANTS (Somatic F0 Knock-Out)

At ZeClinics we design sgRNAs with high specificity, permitting almost 100% efficiency

Specific sgRNA guides unspecific Cas9 endonuclease. By designing the right sgRNAs, interruption at the desired locus of interest is given. Error-prone mechanisms of DNA-repair can cause the generation of small insertions and deletions (INDELs) potentially disrupting the coding sequence of the targeted gene and promoting the formation of loss-of-function mutations.