ZeGenesis – Generation of Genetic Models

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CRISPR/Cas9 Knock-out Models

At ZeClinics we work with the original CRISPR/Cas9 license from the Broad Institute, MIT, and Harvard University to provide the following solutions:

  • Single Knock-Out lines (mutation of one gene)
  • Double Knock-Out lines (simultaneous mutation of two genes, e.g. two paralogues)
  • CRISPANTS (Somatic F0 Knock-Out)

At ZeClinics we design sgRNAs having high specificity and efficiency to guarantee a mutagenesis rate close to 100%.

Figure. Editing a gene using the CRISPR/CAS9 technique. Adapted from Nature News. Carl Zimmer.

A specific sgRNA guides the Cas9 endonuclease to the desired locus of interest to induce targeted double-strand breaks. Error-prone mechanisms of DNA-repair can cause the generation of small insertions and deletions (INDELs) potentially disrupting the coding sequence of the targeted gene and promoting the formation of loss-of-function mutations.