ZeTox – Toxicology Services

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icon-endocrine-disruption-assay Endocrine Disruption Assay Services

Endocrine-disrupting chemicals (EDCs) are organic and inorganic compounds that affect a variety of hormone-regulated physiological pathways in humans and wildlife. EDCs are produced as plasticizers, pesticides, detergents, and pharmaceuticals compounds, and nowadays are widely spread around the globe. Their actions have been associated with reproductive dysfunction, obesity, cancer and developmental anomalies. Because hormone receptors are highly conserved among vertebrates and particularly responsive in fish, zebrafish embryos are an excellent tool for screening EDCs.   

Advantages

Evaluation of disrupting effects in a complete organism.

Specific or simultaneous evaluation of impact in the three major endocrine pathways (estrogens, androgens and thyroid) in only one assay.

Applicable to a wide range of compounds, including mixtures. 

Determination of anti-estrogenic and anti-androgenic effects.

Method description

Zebrafish embryos are exposed to the molecule of interest and the three endocrine pathways are assessed individually or simultaneously through transcriptional analysis of a panel of 8 validated gene expression biomarkers. The disruption can be evaluated as activation or repression of the pathway.

Readouts

Determination of the following activities measured as fold induction compared to controls at 120 hpf:

  • Estrogenicity
  • Anti-estrogenicity
  • Androgenicity
  • Anti-androgenicity
  • Thyroid disruption
Estrogenic and androgenic disruption measured as fold induction compared to the control (E3 medium+DMSO).

References

  1. Jarque S, Ibarra J, Rubio-Brotons M, García-Fernández J, Terriente J. Multiplex Analysis Platform for Endocrine Disruption Prediction Using Zebrafish. Int J Mol Sci. 2019 Apr 8;20(7):1739.