ZeTox

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icon-fast-acute-toxicity Fast Acute Toxicity

There are multiple studies validating zebrafish as predictive model for acute toxicity effects in humans (1-4).

ZeClinics has analyzed the general toxicity of a large set of molecules with known toxic profile in mammals. This analysis shows a significant correlation (Pearson correlation=0.705) between zebrafish LC50 and rodent LD50.

Figure: Correlation between LC50 in zebrafish (log LC50) and LD50 in rodents with intravenuous administration (log_rodent_iv). The associated Pearson Correlation is 0.705

ZeClinics provides different assays to analyze the acute general toxicity of chemicals

Method description

Incubation of embryos with 5 different concentrations in a logarithmic progression of the molecule of interest from 0 to 96 hpf (OCDE No 179, 236).

Readouts

Determination of LOEC, LC50 and BMD values at 24 and 96 hpf, and main parameters of teratogenicity under the microscope at 96 hpf:

  • Mortality
  • Body deformity
  • Developmental delay
  • Scoliosis
  • Pigmentation
  • Edema and heart edema

Mortality of zebrafish larvae exposed to increasing concentration of a toxic agent at two different timepoints

References

  1. McAleer MF, Davidson C, Davidson WR, Yentzer B, Farber SA, et al. 2005. Novel use of zebrafish as a vertebrate model to screen radiation protectors and sensitizers. Int. J. Radiat. Oncol. Biol. Phys. 
  2. Augustine-Rauch K, Zhang CX, Panzica-Kelly JM. 2010. In vitro developmental toxicology assays: a review of the state of the science of rodent and zebrafish whole embryo culture and embryonic stem cell assays. Birth Defects Res.
  3. Brannen KC, Panzica-Kelly JM, Danberry TL, Augustine-Rauch KA. 2010. Development of a zebrafish embryo teratogenicity assay and quantitative prediction model. Birth Defects Res.
  4. Shaukat Ali, Harald G. J. van Mil, Michael K. Richardson. 2011. Large-Scale Assessment of the Zebrafish Embryo as a Possible Predictive Model in Toxicity Testing. PlosOne.