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Drug-induced liver injury is a major cause of attrition during both the early and late stages of drug development and postmarketing. Zebrafish liver is fully developed and functional by 5 days post fertilization. Moreover, metabolic pathways and detoxification mechanisms are similar to those found in humans. The use of zebrafish larvae for the assessment of compound-induced liver toxicity allows to streamline the process of drug evaluation and reduce the risk of drug attrition in the following phases.
3Rs-aligned method for high throughput in vivo hepatotoxicity screening.
High concordance between zebrafish and mammals after exposure to hepatotoxic and non-hepatotoxic compounds.Bridge between cell-based and mammalian methods with results in short times.Simultaneous assessment of cardiotoxicity and neurotoxicity through the service ZeGlobalTox.
High concordance between zebrafish and mammals after exposure to hepatotoxic and non-hepatotoxic compounds.
Bridge between cell-based and mammalian methods with results in short times.
Simultaneous assessment of cardiotoxicity and neurotoxicity through the service ZeGlobalTox.
Transgenic zebrafish larvae expressing red fluorescent protein in the hepatocytes are incubated with the No Observable Effect Concentration (NOEC) of the compound of interest from 96 to 120 hours post fertilization (hpf). The fluorescent signal is used to measure the hepatic area. Specific fat staining is later performed to quantify the lipid content in the yolk and in the liver.
Evaluation of hepatic morphological and functional parameters at 120 hpf:
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