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BY THERAPEUTIC AREA
BY OUR PLATFORMS
As an officially CRISPR licensed provider, ZeClinics can permanently inactivate your gene of interest in zebrafish to generate tailored knock-out (KO) lines from 6/7 months. This approach is the best option for a fine characterization of a gene’s biological function, for disease modeling, and for drug screening in the context of a disease.
Using the CRISPR/Cas9 system in zebrafish, we can disrupt the CDS of a gene or the integral regulatory domains (KO null), or remove the entire functional domain or the entire CDS depending on gene size (KO deletion).
This technique can be used to generate single KO lines (mutation of one gene) or double KO lines (mutation of two genes, e.g. two paralogues).
Get the edit you want along with the support you need to start your KO project.
However, if speed is what you're after, ZeClinics' Crispants offer a fast-track option for target validation.
Highly efficient mutagenesis (close to 100%)Extreme flexibility of the CRISPR/Cas9 system that allows customized generation of zebrafish linesHigh rate of success in KO generation (99%)
Highly efficient mutagenesis (close to 100%)
Extreme flexibility of the CRISPR/Cas9 system that allows customized generation of zebrafish lines
High rate of success in KO generation (99%)
We design sgRNAs having high specificity and efficiency to guarantee a mutagenesis rate close to 100%.
Specific sgRNA guide the Cas9 endonuclease to the desired locus of interest to induce targeted double-strand breaks. Error-prone mechanisms of DNA-repair can cause the generation of small insertions and deletions (INDELs) potentially disrupting the coding sequence of the targeted gene and promoting the formation of loss-of-function mutations. Alternatively, a sequence of interest can also be completely deleted.
If you want more information about our knock-outgeneration services or have any other questions, please contact our experts.