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Gene knock-out (KO) is a genetic technique employed to inactivate one of an organism’s genes. The creation of stable KO zebrafish lines ensures to obtain isogenic lines in which every fish carries the same mutation in the gene of interest. This approach is the best option if the candidate target gene has already been identified and the aim of the experiment is to achieve a fine characterization of its biological function, generate a disease model or perform a drug screening.
This technique can be used for generating single KO lines (mutation of one gene) or double KO lines (mutation of two genes, e.g. two paralogues).
According to your needs, either F1 heterozygous KOs (carrying one mutated allele) or F2 homozygous KOs (with two mutated alleles) can be generated and shipped worldwide.
Highly efficient mutagenesis (close to 100%)Extreme flexibility of the CRISPR/Cas9 system that allows customized generation of zebrafish linesHigh rate of success in KO generation (99%)
Highly efficient mutagenesis (close to 100%)
Extreme flexibility of the CRISPR/Cas9 system that allows customized generation of zebrafish lines
High rate of success in KO generation (99%)
We are an officially licensed CRISPR solutions provider. We design sgRNAs having high specificity and efficiency to guarantee a mutagenesis rate close to 100%.
A specific sgRNA guides the Cas9 endonuclease to the desired locus of interest to induce targeted double-strand breaks. Error-prone mechanisms of DNA-repair can cause the generation of small insertions and deletions (INDELs) potentially disrupting the coding sequence of the targeted gene and promoting the formation of loss-of-function mutations.