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Exposure to some drugs might alter the normal activity of the nervous system and even cause irreversible damage to the nervous tissue. When evaluating neurotoxic effects, we differentiate between CNS side effects, referring to functional and transient alterations, such as sedation, and neurotoxicity itself considering neuronal loss or permanent structural changes. These adverse effects are widespread and amongst the most frequent reasons for drug attrition.
NeuroTox takes advantage of the high similarity between zebrafish and human nervous system structure and function to predict the harmful consequences of new compounds exposure or custom gene mutations on the nervous system.
3Rs-aligned method for high throughput in vivo neurotoxicity screening.
Assessment of a wide myriad of behavioral phenotypes such as feeding, seizures, involuntary movement, sleeping, and addiction.Customizable experimental design.Evaluation of potential neurotoxicity protective candidates.Simultaneous assessment of cardiotoxicity and hepatotoxicity through the service ZeGlobalTox.
Assessment of a wide myriad of behavioral phenotypes such as feeding, seizures, involuntary movement, sleeping, and addiction.
Customizable experimental design.
Evaluation of potential neurotoxicity protective candidates.
Simultaneous assessment of cardiotoxicity and hepatotoxicity through the service ZeGlobalTox.
Transgenic zebrafish larvae expressing green fluorescent protein (GFP) in a neuronal transgenic reporter are incubated with 1-5 concentrations of the compound of interest based on the previously determined BMD. According to the exposure timeframe, we would be able to measure:
Locomotor activity in response to light/dark cycles is monitored using the Daniovision™ device together with the Ethovision XT software (Noldus IT). Behavioral alterations are subsequently associated with specific drug-induced neuronal impact. Optionally, larvae can be then fixed and neuronal clusters observed to determine changes in neuronal number. In addition to basal locomotor activity, other behavioral responses such as seizures, thigmotaxis, and sedation are evaluated as CNS side effects.
Determination of behavioral phenotypes:
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