ZeTox – Toxicology Services

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icon-neuro-tox NeuroTox – Neurotoxicity Service

Exposure to some drugs might alter the normal activity of the nervous system, even promoting irreversible damage to nervous tissue. Neurotoxicity is remarkably common and among the most frequent reasons for drug attrition. 

Advantages

Zebrafish is an excellent model to assess a myriad of behavioral phenotypes: feeding, seizures, involuntary movement, sleeping, and addiction.

Evaluation of the neurotoxic effect of compounds assessed through behavioral phenotyping.

Simultaneous assessment of cardiotoxicity and hepatotoxicity through the service ZeGlobalTox.

Method description

High-throughput tracking of zebrafish larvae movements is automated using the DanioVision™ system (Noldus IT).  Locomotor activity of zebrafish larvae is assessed upon the alternation of Light and dark phases during the trial. Alteration in total locomotion and/or its stereotyped pattern suggests a drug-induced neuronal impact.

ZeClinics neurotoxicity assay has been internally validated through the analysis of a 24 compounds library.

Readouts

  • Locomotor activity 
  • Stereotyped pattern
  • Neuronal survival. A transgenic line with fluorescent reporter protein is used for these experiments. 

References

  1. Cornet C, Calzolari S et al. 2017. ZeGlobalTox: an innovative approach to address organ drug toxicity using zebrafish. Int. J. Mol. Sci.  18, 864
  2. Parng, C. et al. 2007. Neurotoxicity assessment using zebrafish. J. Pharmacol.Toxicol. Methods.
  3. Kokel D, Bryan J, Laggner C, White R, Cheung CY, et al. 2010. Rapid behavior-based identification of neuroactive small molecules in the zebrafish. Nat. Chem. Biol.
  4. Lam CS, Korzh V and Strahle U. 2005. Zebrafish embryos are susceptible to the dopaminergic neurotoxin MPTP. Eur. J. Neurosci.