Somatic knock-out (Crispants)

The injection of zebrafish one-cell-stage embryos with highly active Cas9/sgRNAs RNP complexes promotes genomic mutations at the desired site with very high efficiency (up to 100%).

In vitro assembled sgRNA/Cas9 ribonucleoprotein complexes speed up the formation of double strand breaks (DSBs)

Thanks to this extremely high performance, at ZeClinics we are able to generate somatic mutants (Crispants) carrying a high rate of biallelic mutations in F0 animals.

This approach ensures the creation of “de facto” F0 mutants enabling the evaluation of loss-of-function phenotypes in short time (2-3 months).

Genotyping of mosaic F0 larvae permit associating mutation rates with resulting phenotypes.