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ZeClinics’ knock-down (KD) approaches are a fast and very easy way to see if your gene of interest might have a phenotype (reverse genetic analysis), and warrant making a germline mutant.
Our genetic experts can generate functional KDs of gene expression in F0 animals using antisense oligonucleotides (ASOs). This procedure does not compromise the genomic DNA but alters the transcribed mRNA.
Transient gene KD will help establish a direct link between the biochemical function of a gene product and its role in vivo. Phenotyping is possible with good controls but the decreasing concentration of ASOs leads to sub-optimal KD usually by 4dpf.
Looking for a fast strategy to find what phenotypes are controlled by your genes of interest?
The main difference of this technique compared to other alternatives for fast target validation, such as Crispants, is the transient nature of the genetic modulation.
Scalable solution: high throughput screening of multiple candidate genesConfirmation of the phenotypic output before establishing the KO lineNon-invasive, in vivo readouts to detect KO-induced phenotypes at the level of cells, tissues or organs
Scalable solution: high throughput screening of multiple candidate genes
Confirmation of the phenotypic output before establishing the KO line
Non-invasive, in vivo readouts to detect KO-induced phenotypes at the level of cells, tissues or organs
ASOs are RNA-DNA hybrid oligonucleotides binding the target mRNAs to induce their degradation via RNAse H or interfere with their maturation/translation. The resulting phenotypes are transient, as larvae tend to recover as ASOs get degraded. For this reason, ASOs-based approaches are better suited to study phenotypes arising during the early stages of embryonic development.
Results of the phenotypic analysis according to customers' interests and needs:
If you want more information about our ASO-basedknock-down services or have any other questions,please contact our experts.