ZeGenesis – Genetic Services

BY
OUR
PLATFORMS

antisense-oligonucleotides-icon Antisense oligonucleotides-based knock-down

We can study the function of genes of interest by performing knock-down (KD) experiments based on the use of antisense oligonucleotides (ASO). This approach does not compromise the genomic DNA but alters the transcribed mRNA.

Applications

  • Pre-clinical evaluation of ASO’s- based therapies
  • Temporary inhibition of gene-expression at early developmental stages
  • Cost-effective screening of candidate genes that are suspected to be involved in a given disease

Advantages

Fast target validation prior to the generation of stable loss-of-function alleles

High throughput screening of multiple candidate genes

Non-invasive, in vivo readouts to detect KO-induced phenotypes at the level of cells, tissues or organs

Method description

ASOs are RNA-DNA hybrid oligonucleotides binding the target mRNAs to induce its degradation or interfere with its maturation/translation. Resulting phenotypes are transient, as larvae tend to recover as ASOs get degraded. For this reason, ASOs-based approaches are better suited to study phenotypes arising during the early stages of embryonic development.

Figure 1. Transient KD induced by the injection of ASOs into one-cell-stage embryos. The levels of ASOs in zebrafish tissues decrease over time upon injection.

Readouts

Results of the phenotypic analysis according to customers' interests and needs:

  • Teratogenic phenotypes
  • Organ-specific morphological or functional defects (heart-specific, liver-specific etc.)
  • Behavioral phenotypes

References

  1. Pauli A, Montague TG, Lennox KA, Behlke MA, Schier AF. Antisense Oligonucleotide-Mediated Transcript Knockdown in Zebrafish. PLoS One. 2015 Oct 5;10(10):e0139504.