BY OUR PLATFORMS
BY THERAPEUTIC AREA
BY OUR PLATFORMS
ZeGenesis platform is the most up-to-date genetic toolbox for highly efficient mutagenesis, transgenesis, and gene expression modulation in zebrafish.
We are an officially licensed CRISPR solutions provider under the Broad Institute of MIT/Harvard, and specialists in Tol2 and other genome editing tools.
Using these advanced technologies, our experts in zebrafish genome editing can generate tailored knock-out, knock-in, and transgenic lines. We can also transiently modify gene expression according to your experimental needs.
We collaborate with you on the project design, to make sure you get the zebrafish genome edit that best fits your experimental needs the soonest time feasible.
Our zebrafish genetic services are well-suited for reverse genetic studies. Reverse genetics seeks to find what phenotypes arise from particular engineered genetic sequences. The final aim of these approaches can be:
Understanding gene function in the developing or adult zebrafish.
Genome engineering models are essential tools in life science research, in order to faithfully establish human disease models.
In vivo validation of candidate genes in the context of a disease, for druggable target identification and subsequent drug design.
Investigating the efficacy of candidate therapeutic compounds in the context of a disease.
Zebrafish genetic models can also be valuable tools to study biological processes. We can establish tissue- and time-specific reporter lines to study organism development, morphology, and function. Transcriptional reporters allow monitoring the evolution of a biological process.
It is a fully sequenced vertebrate.It shares 82% of human disease-related genes.It has a high number of genes (41,154) and number of protein coding (26,373 vs 22,493 in mouse).It is amenable to genetic manipulation, including reverse genetic approaches.CRISPR-Cas9 system has proven to be a highly specific, highly efficient and reliable approach for genome editing in zebrafish.Zebrafish embryos allow high-throughput screening of pathological phenotypes in a whole-living animal.The use of genetically modified zebrafish has already allowed the identification of new putative therapeutic drugs.
It is a fully sequenced vertebrate.
It shares 82% of human disease-related genes.
It has a high number of genes (41,154) and number of protein coding (26,373 vs 22,493 in mouse).
It is amenable to genetic manipulation, including reverse genetic approaches.
CRISPR-Cas9 system has proven to be a highly specific, highly efficient and reliable approach for genome editing in zebrafish.
Zebrafish embryos allow high-throughput screening of pathological phenotypes in a whole-living animal.
The use of genetically modified zebrafish has already allowed the identification of new putative therapeutic drugs.
Our experts will give you the advice to select the best genetic approach according to your needs. Our goal is to assist you in moving forward with your experiments and shorten the period between project conception and completion.
Due to a lack of:
Explore our offerings:
Stable genome editing approaches for loss-of-function mutagenesis and random or target insertion of exogenous DNA. You can choose from:
Alternative more rapid approaches to evaluate the effect of gene loss-of-function or gene overexpression at early developmental stages. To this end, we provide you with different solutions:
We offer a variety of service packages to fit the needs and budget of your lab. Our offerings can be fully customized, enabling you to adapt the assistance you require to achieve your gene editing goals.
We create and accurately validate your custom injection mix and deliver expertly injected embryos ready to submit to your nursery.
It includes the entire process of design, sequence full validation, injection, rearing, germline screening, and line propagation up to F1 generation to deliver stable transgenic/knock-out/knock-in zebrafish lines.
It includes the entire process of design, sequence full validation, injection, rearing, germline screening, and line propagation up to F2 or F3 generation to deliver stable transgenic/knock-out/knock-in zebrafish lines.
*In order to receive our genetically modified zebrafish embryos, facilities for zebrafish growth, maintenance, and crossing are needed.
In combination with our ZeEfficacy platform, zebrafish genetic models can be phenotypically characterized or used for compound screening. Thereby, we offer:
End-to-end reverse genetic studies. We phenotypically characterize your transgenic, knock-out and knock-in zebrafish lines.
Fast-track choice for reverse genetics. We create and accurately validate your custom injection mix, and phenotypically characterize somatic F0 mutants.
We use your custom genetically modified lines for efficacy screening of candidate therapeutic compounds.
If you want more information about our geneticservices or have any other questions, pleasecontact our experts.