Genetic modifications are needed for different scientific purposes: i) in order to validate the cause of a disease, ii) to create a model for drug-screening or iii) to perform functional genomic studies.

Large genetic disease variability and limitations of murine models open the need for innovative models in which mutants can be easily generated and quickly validated by phenotypic evaluation.

Zebrafish allow specific and highly efficient transgenesis and mutational studies, with capability to generate a high number of economic and highly informative genetic lines, as well as direct-phenotypes; thanks to high efficiency and the ability to manipulate individual fertilized eggs. Moreover, phenotypes of the resulting diseases can be characterized in different manners