ZeGenesis – Genetic Services

ZeGenesis: Customized genomics

ZeGenesis platform is the most up-to-date genetic toolbox for highly efficient mutagenesis, transgenesis, and gene expression modulation in zebrafish.

We are an officially licensed CRISPR solutions provider under the Broad Institute of MIT/Harvard, and specialists in Tol2 and other genome editing tools.

Using these advanced technologies, our experts in zebrafish genome editing can generate tailored knock-out, knock-in, and transgenic lines. We can also transiently modify gene expression according to your experimental needs.

Scientific purposes

Our zebrafish genetic services are well-suited for reverse genetic studies. Reverse genetics seeks to find what phenotypes arise from particular engineered genetic sequences. The final aim of these approaches can be:

Zebrafish genetic models can also be valuable tools to study biological processes. We can establish tissue- and time-specific reporter lines to study organism development, morphology, and function. Transcriptional reporters allow monitoring the evolution of a biological process.

Why zebrafish?

It is a fully sequenced vertebrate.

It shares 82% of human disease-related genes.

It has a high number of genes (41,154) and number of protein coding (26,373 vs 22,493 in mouse).

It is amenable to genetic manipulation, including reverse genetic approaches.

CRISPR-Cas9 system has proven to be a highly specific, highly efficient and reliable approach for genome editing in zebrafish.

Zebrafish embryos allow high-throughput screening of pathological phenotypes in a whole-living animal.

The use of genetically modified zebrafish has already allowed the identification of new putative therapeutic drugs.

How to choose our services?

Our experts will give you the advice to select the best genetic approach according to your needs. Our goal is to assist you in moving forward with your experiments and shorten the period between project conception and completion.

Types of edits

Transgenesis and CRISPR gene editing

Transgenic linesKnock-out linesCrispants: Fast-track KOKnock-in lines

Transient Knock-down and overexpression approaches

Alternative more rapid approaches to evaluate the effect of gene loss-of-function or gene overexpression at early developmental stages. To this end, we provide you with different solutions:

Morpholino-based knock-downAntisense oligonucleotides-based knock-downTransient overexpression

Service packages

We offer a variety of service packages to fit the needs and budget of your lab. Our offerings can be fully customized, enabling you to adapt the assistance you require to achieve your gene editing goals.

Deliverables

Injected Embryos

We create and accurately validate your custom injection mix and deliver expertly injected embryos ready to submit to your nursery.

Heterozygous Line

It includes the entire process of design, sequence full validation, injection, rearing, germline screening, and line propagation up to F1 generation to deliver stable transgenic/knock-out/knock-in zebrafish lines.

Homozygous Line

It includes the entire process of design, sequence full validation, injection, rearing, germline screening, and line propagation up to F2 or F3 generation to deliver stable transgenic/knock-out/knock-in zebrafish lines.

*In order to receive our genetically modified zebrafish embryos, facilities for zebrafish growth, maintenance, and crossing are needed.

Phenotypic assessments

In combination with our ZeEfficacy platform, zebrafish genetic models can be phenotypically characterized or used for compound screening. Thereby, we offer: