ZeGenesis – Genetic Services

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ZeGenesis: Customized genomics

The ZeGenesis platform is a portfolio of genetic services including but not limited to mutagenesis, transgenesis, and gene expression modulation studies. We are an officially licensed CRISPR solutions provider

We can generate tailored somatic or germline editing in order to deliver stable genetically-modified zebrafish lines optimized to your requirements. We can also transiently modify gene expression according to your experimental needs.

Our zebrafish genetic services are the solution for the following scientific purposes:

  • Study of biological processes
  • Disease modeling
  • Genetic functional studies
  • Target gene validation
  • Therapeutic compounds screening in a given condition

The Zebrafish model has emerged as an alternative to in vitro and murine models that allows mutants and transgenics to be more easily generated and phenotypically analyzed. These are some of its advantages:

  • Small size, large progeny and fast life cycle turn zebrafish into a cost-effective genetic model
  • Specific and highly efficient mutagenesis and transgenesis allow high throughput generation of highly informative genetic lines
  • High genetic homology between zebrafish and humans enables the generation of loss- or gain-of-function models for 82% of human disease-causing genes
  • High structural and functional conservation between zebrafish and mammals has positioned the model as an ideal tool for the study of diseases, biological processes and drug effects
  • The optical transparency of zebrafish embryos makes them the perfect tool to work with tissue-specific-reporter lines in vivo

Our offerings

Transgenesis and gene editing services

According to the scientific questions being asked, you may require the generation of stable genetically-modified lines. To this end, we can establish and deliver your customized stable mutant or transgenic zebrafish strains carrying germline edits. You can choose from:

These stable lines are the perfect approach for:

  • Generating humanized disease models
  • Establishing tissue-specific reporter lines
  • Creating environmental sensors such as optogenetic and chemogenetic tools
  • Generating transcriptional reporters to monitor the evolution of a biological process

In combination with our ZeEfficacy platform, we can additionally perform phenotypic characterization of these lines or use them for compound screening.

Transient Knock-down and overexpression approaches

If your biological question requires the analysis of the effect of gene loss-of-function or gene overexpression at early developmental stages, the generation of stable genetically-modified zebrafish lines may not be required.

In these cases, we offer alternative more rapid approaches, which consist of transiently modifying gene expression. This strategy is also appropriate if you want to evaluate a set of candidate genes in order to discard invalid targets and prioritize the best options.

To this end, we provide you with different solutions: 

Phenotypes resulting from gene loss-of-function or gene overexpression can be characterized on demand in the whole organism or in specific organs.