ZeEfficacy – Efficacy Services


Organ-specific assessments

Our extensive portfolio of phenotyping assays in zebrafish provides a path to evaluate, both in the whole organism and specific organs, phenotypes resulting from pharmacological treatment and/or gene variation, for lead therapeutics identification and disease biology understanding.

Phenotype-based screening is one of the most effective approaches to drug discovery. The Zebrafish model allows to perform in vivo efficacy tests cost-effectively, with high biological translatability and reduced ethical constraints. Thanks to their optical transparency, zebrafish embryos allow easy and non-invasive evaluation of morphological, functional, biochemical and behavioral features. Specific phenotypes can also be assessed in adult fish, especially for evaluations over an extended period of time.

Whole organism
  • Survival/mortality
  • Body weight
  • Determination of growth rate
  • General morphological abnormalities
  • Teratogenic index
  • Peptide bioavailability
  • Inflammation studies (neutrophils and macrophages recruitment)
  • Heart function
  • Cardiac malformations
  • Liver necrosis
  • Hepatomegaly
  • Yolk lipid retention
  • Steatosis: fat accumulation in the liver
  • Muscle integrity
  • Muscle functionality (locomotion)
Inner ear
  • Auditory system functionality (behavioral response to acoustic stimulus)
  • Hair Cell (HC) survival & functionality
  • HC morphology analysis
  • Otolith formation
Central nervous system
  • Locomotion activity and patterns
  • Visual response
  • Thigmotaxis
  • Habituation
  • Convulsive behavior (seizures)
  • Specific neuronal population analysis
  • Glucose evaluation
  • Cholesterol accumulation
  • Angiogenesis (new blood vessels generation)
  • Intestine morphology
  • Intestine motility/functionality
  • Tissue-specific cell-based analysis
  • Fertility rate
  • Male gonads weight
  • Tissue-specific cell-based analysis: retinal photoreceptors survival & regeneration