ZeEfficacy – Efficacy Services


Epilepsy Model

Epilepsy is characterized by recurrent, unprovoked seizures that result from excessive and hypersynchronous electrical discharges in the brain.

Exposure to the common convulsant agent pentylenetetrazole (PTZ) induce a stereotyped and concentration-dependent sequence of behavioral changes culminating in convulsions in zebrafish larvae. 

Zebrafish has demonstrated to be a powerful system to study the causes and mechanisms of seizures and identify new anti-convulsive drugs.

Method description

Embryos are exposed to the compound of interest, together with PTZ to unchain seizures.  Anti-seizure controls compare activity. 

Behavior and seizures are assessed using the following scheme to evaluate the anti-convulsant properties of compounds:

Figure: Experimental design


  • Locomotion activity: Total distance moved, locomotion pattern and visual stimuli response.
  • Convulsive response: Seizures can be evaluated as a response to a flashlight. These are evaluated though velocity and angle turn parameters.
Pentyleneterazole (PTZ) produces seizures that can be reverted by the anti-convulsive compounds Topiramate and Valproic Acid.