ZeEfficacy – Efficacy Services


Retinal Degeneration Model

Dry-AMD leads to gradual reduction of vision, characterized by progressive loss of the photoreceptor cell population in the retina

High anatomic and functional conservation between human and zebrafish retina make adult zebrafish models very suitable.

Disruption of photoreceptors in adult zebrafish retinae are achieved through constant, intense light irradiation.

Zebrafish are a comprehensive and biological translatable method to evaluate the efficacy of Dry age-related macular disease (Dry-AMD) therapies

Figure: Retinal Degeneration Model

The evaluation of the compounds is based on the analysis of the thickness and cell number of the Outer Nuclear Layer (ONL), the retinal layer populated by rod and cone photoreceptors. 

Cryosections of the eyes are immuno-stained against a photoreceptor marker, in order to evaluate this cell population as shown in the figure below.

Figure: Specific assay result of Retinal Degeneration Model