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Dry Age-related Macular Disease (Dry-AMD) is a common eye disorder leading to a gradual reduction of vision, characterized by progressive loss of the photoreceptor cell population in the retina.
Given the high anatomic and functional conservation between human and zebrafish retina, adult zebrafish are very suitable to model human eye disorders.
We offer a comprehensive and biologically translatable method to study human retinal degeneration pathogenesis and evaluate the efficacy of Dry-AMD therapies.
Zebrafish and human retina are highly conserved both anatomically and functionally.The fast development of the eye.Zebrafish retina has the long-life ability to regenerate upon injury, allowing screening of compounds potentially accelerating tissue recovery.Transparency of the model.
Zebrafish and human retina are highly conserved both anatomically and functionally.
The fast development of the eye.
Zebrafish retina has the long-life ability to regenerate upon injury, allowing screening of compounds potentially accelerating tissue recovery.
Transparency of the model.
The assay is divided into different phases. The order of the phases depends on the aim of the experiment.
Neural retina protection assay
For testing the compound’s capacity to protect from degeneration, the order of phases is the following:
Neural retina regeneration assay
For testing pro-regenerative compounds, the order is the following:
In both cases, treated and control eyes are enucleated and fixed. Cryosections of the eyes are immuno-stained against a photoreceptor marker to evaluate this cell population. Imaging is done by confocal microscopy, and analysis is performed through a custom image analysis software that allows process automation and unbiased analysis between samples.