ZeEfficacy – Efficacy Services

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Getting the right insights

ZeEfficacy platform is a portfolio of services including high throughput phenotypic screening and disease models for in vivo target validation in zebrafish. 

Zebrafish respond to drug treatments at physiologically relevant dose ranges. We can assess cost-effectively the activity of your molecule within the complexity of a whole organism and across tissues by phenotypic drug screening.

When combined with our genetic services, these phenotypic approaches will allow you to:

  • Test candidate genes: the function of a given gene can be determined by evaluating the effect of a genetic modification on specific phenotypes.
  • Validate a disease model: checking if the target gene editing recapitulates all the relevant pathological traits of the modelled disease.
  • Screen for therapeutic compounds: identifying those molecules that rescue the wild-type phenotype in a validated disease model.

Zebrafish, a highly informative model

Zebrafish has been revealed as a powerful in vivo system for modeling human diseases and drug discovery. Among its advantages can be found: 

High homology: zebrafish have a similar genetic structure to humans.  More than 70% of human genes have a zebrafish orthologue gene. This percentage goes up to 82% if we only consider genes that are associated to human diseases.

High conservation: most zebrafish and human genes have a conserved function. As a result, most organs have a comparable histological organization and sustain the same function in the two species.

Biological translatability of pathological phenotypes to human disease biology. Zebrafish’s disease characteristics, aetiology and progression, and molecular mechanisms are highly conserved and clinically relevant.

Custom Phenotyping

We provide an array of high throughput phenotypic characterization assays for pharmacological and gene validation. These studies will enable you to rapidly address, in a whole organism, the consequences of drug treatment or genome editing, in order to identify impactful drugs and disease-relevant genes.

Phenotype-based screening is one of the most effective approaches to drug discovery. Zebrafish allows performing in vivo efficacy tests cost-effectively, with a high biological translatability and reduced ethical constraints. Thanks to its optical transparency, zebrafish embryos allow easy and non-invasive evaluation of morphological, functional, biochemical and behavioral features. Specific phenotypes can also be assessed in adult fish, especially for evaluations over an extended period of time.

Our imaging and high throughput capabilities, plus our expertise in several therapeutic areas, allow us to offer a wide range of organ-specific analyses, which can be customized to suit your needs:

Disease models

We are specialized in the generation of zebrafish disease models to be used in early stages of drug discovery. You can take advantage of our ready-to-use models covering multiple research fields, or ask for your tailored-made disease model on a specific therapeutic area. These humanized model systems can serve as efficient tools to study human diseases and to screen for potential therapeutic compounds.

Unlike cell-based platforms, zebrafish permits modeling a wider range of disease-associated phenotypes, can better mimic the genetic heterogeneity of human patients, and physiological outputs of human conditions.