ZeGenesis – Generation of Genetic Models


Morpholino-based Knock-down Lines

Knock-down experiments are based on the use of morpholino oligonucleotides (MO). This approach does not compromise the genomic DNA but alters the transcribed mRNA.

Figure: Morpholino oligonucleotides (MO)

MOs binds the target mRNAs, blocking the synthesis of the proteins encoded by the targeted mRNAs.

Resulting phenotypes are transient, larvae tend to recover as MO activity decreases and protein production re-establishes.