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Zebrafish (Danio rerio) is a small tropical freshwater fish, native to the streams of the southeastern Himalayan region that commonly inhabits slow-moving water bodies. It takes his name from the horizontal blue-pigmented stripes aligning his body, which are reminiscent of the zebra stripes. Danio rerio adults grow up to 4 cm, and their lifespan is between two to three years in standard laboratory conditions. Both size and age can be larger in their natural habitat.
Zebrafish are omnivorous, primarily eating zooplankton and insects, although they can eat a variety of other foods, such as worms, small crustaceans or algae. This is one of the reasons that make this freshwater fish an extremely robust animal model.
The approximate sexual maturity time for them is three months. A male must be present to induce female ovulation and egg fecundation. Females can spawn at intervals of two to three days, laying more than two hundred eggs in each mating. This vast progeny is another advantage of using this particular family of fishes.
Upon eggs release, embryonic development begins. The zebrafish embryo develops quickly, with precursors to all significant organs appearing within 36 hours post-fertilization (hpf). The embryo starts as a yolk with a single enormous cell on top, which divides into two and continues dividing until there are thousands of small cells. The cells then migrate down the sides of the yolk and begin forming a head and tail, which later grows and separates from the body. From 4 dpf, heart physiology is stable; from 5 dpf they respond to optical, touch and acoustic stimuli and display free swimming, which suggests a sophisticated nervous system; also from 5 days, the digestive/excretory system is fully functional. Interestingly, zebrafish embryonic and
larval tissues are optically transparent, which allows visualizing morphogenetic movements and organs in vivo (i.e., Heart beating). Thus, the fast life cycle and transparency are additional features making this species a robust animal model for biomedicine.
All the named advantages have allowed zebrafish to become an essential tool for high-throughput screenings of small molecules with therapeutic effects. In that sense, both academy and pharmaceutical industry use zebrafish to save time and money in preclinical studies for assessing toxicity and efficacy of new drugs, in the fields of infection, cardiovascular diseases, neural disorders or cancer. In summary, the advantages of using an animal model such as zebrafish for biomedicine and drug discovery are multiple:
All these features have allowed the use of zebrafish to yield advances in the fields of developmental biology, oncology, toxicology, reproductive studies, teratology, genetics, neurobiology, environmental sciences, stem cell research and regenerative medicine, and evolutionary theory… and this is only the beginning!